Staphylococcal enterotoxin B-derived haptens promote sensitization

Neutralization of Staphylococcal enterotoxin B by aptamer

12 Staphylococcal enterotoxin B (SEB) is a major virulence factor for 13 Staphylococcal toxic shock syndrome (TSS). SEB activates a large subset of the T 14 lymphocytic population, releasing pro-inflammatory cytokines. Blocking 15 SEB-initiated toxicity may be an effective strategy for treating TSS. Using a 16 systematic evolution of ligands by exponential enrichment (SELEX) process, we 17 iden...

Purification of Type B Staphylococcal Enterotoxin.

Frea, James I. (University of Wisconsin, Madison), Elizabeth McCoy, and F. M. Strong. Purification of type B staphylococcal enterotoxin. J. Bacteriol. 86:1308-1313. 1963.-A procedure for the production and isolation of type B staphylococcal enterotoxin in yields up to 4 mg per liter of toxic culture is presented. Staphylococcus aureus S6 was grown in shake flask cultures at 37 C for 24 hr in a ...

A DNA Spiegelmer to staphylococcal enterotoxin B.

Bacterial staphylococcal enterotoxin B is involved in several severe disease patterns and it was therefore used as a target for the generation of biologically stable mirror-image oligonucleotide ligands, so called Spiegelmers. The toxin is a 28 kDa protein consisting of 239 amino acids. Since the full-length protein is not accessible to chemical peptide synthesis, a stable domain of 25 amino ac...

Staphylococcal Enterotoxin B and Related Pyrogenic Toxins

Hydrogen ion equilibrai of staphylococcal enterotoxin B.

Potentiometric and spectrophotometric titrations of staphylococcal enterotoxin B have been carried out in 0.16 M KC1 at 25”. Potentiometric titration results indicate that the titration curves are essentially reversible between pH 1.50 and 11.0. All of the ionizable groups in enterotoxin B found from the titration curves are consistent with the amino acid composition which has been reported in ...